Table 1. Design of multifactorial experiment with 12 treatments testing aggregation of the diatom T. weissflogii in the presence or absence of bacteria at two temperatures and three pCO2 scenarios.
| Treat. # | Temp. °C | pCO2 | Bact. |
| 1 | 15 | Am | Ax |
| 2 | 15 | Am | HP |
| 3 | 15 | F1 | Ax |
| 4 | 15 | F1 | HP |
| 5 | 15 | F2 | Ax |
| 6 | 15 | F2 | HP |
| 7 | 20 | Am | Ax |
| 8 | 20 | Am | HP |
| 9 | 20 | F1 | Ax |
| 10 | 20 | F1 | HP |
| 11 | 20 | F2 | Ax |
| 12 | 20 | F2 | HP |
Xenic treatments contained the bacterium M. adhaerens HP15. Each treatment was prepared in triplicate; one replicate was harvested initially (t = 0) and two after 96 hr. incubation on roller tables in the dark. See text for specifics on pCO2 treatments. Ax = axenic, HP = M. adhaerens HP15 added, Am = Ambient, F1 = Future 1, F2 = Future 2.