Table 1. Design of multifactorial experiment with 12 treatments testing aggregation of the diatom T. weissflogii in the presence or absence of bacteria at two temperatures and three pCO2 scenarios.
Treat. # | Temp. °C | pCO2 | Bact. |
1 | 15 | Am | Ax |
2 | 15 | Am | HP |
3 | 15 | F1 | Ax |
4 | 15 | F1 | HP |
5 | 15 | F2 | Ax |
6 | 15 | F2 | HP |
7 | 20 | Am | Ax |
8 | 20 | Am | HP |
9 | 20 | F1 | Ax |
10 | 20 | F1 | HP |
11 | 20 | F2 | Ax |
12 | 20 | F2 | HP |
Xenic treatments contained the bacterium M. adhaerens HP15. Each treatment was prepared in triplicate; one replicate was harvested initially (t = 0) and two after 96 hr. incubation on roller tables in the dark. See text for specifics on pCO2 treatments. Ax = axenic, HP = M. adhaerens HP15 added, Am = Ambient, F1 = Future 1, F2 = Future 2.