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. 2014 Nov 6;10(11):e1004462. doi: 10.1371/journal.ppat.1004462

Figure 3. NLRP3-deficient mice exhibit reduced neutrophil recruitment and IL-1β production in pulmonary aspergillosis.

Figure 3

C57BL/6 and Nlrp3−/− mice were infected intranasally with A. fumigatus and treated with 1000 ng/mouse recombinant IL-37 precursor administered intraperitoneally 1 hour before the infection. (A) BAL fluid morphometry [number of total (T) cells and polymorphonuclear neutrophils (P). Values represent the mean±SD of three mice per group and are representative of 3 independent experiments]. (B) Lung histology (periodic acid-Schiff staining) and cell recruitment (insets). Scale bars, 100 µm and 25 µm, respectively. Arrows indicate neutrophils. (C) Mpo and Cxcl2 mRNA expression by RT-PCR on total lung cells. (D) IL-1β production (ELISA on lung homogenates). Assays were done 3 days after the infection. Data are representative (histology) or pooled from three experiments. *P<0.05, Nlrp3−/− vs C57BL/6 mice and treated vs untreated (None) mice. Naïve, uninfected and untreated mice.