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. 2014 Nov 6;9(11):e111914. doi: 10.1371/journal.pone.0111914

Figure 6. Fragment A Enhancer is responsive to BMP4 stimulation but not TGFβ1.

Figure 6

(A) Enhancer activity was measured by luciferase assay for the fragment A containing the major (CGTC) and minor (TCCG) haplotypes in serum starved HCT-116 and SW480 cells incubated with TGFβ1 for 6 hours. Samples are plotted as a fold change in activity relative to non-treated cells on the same plate. (B) Enhancer activity was stimulated by 6 hours of BMP4 treatment for the CGTC haplotype in HCT-116 (p = 4.14×10−3) and SW480 cells (p = 1.22×10−10). The TCCG haplotype in fragment A shows lower levels of stimulation, and only in SW480 (p = 1.61×10−6). (HCT-116 p = 0.38). Effect of BMP is plotted as fold change over untreated samples for each haplotype on the same plate. (C) Enhancer activity comparison between the CGTC and TCCG haplotypes following BMP4 treatment. Relative luciferase activity was plotted for each haplotype in HCT-116 and SW480 using the same experimental dataset as (B).