Figure 2. S. Typhimurium hns mutant lineages acquired large chromosomal deletions in the SPI-1 invasion locus.
Throughout the 30-day evolution period, 5 out 6 Δhns lineages incurred 10–50 kb deletions in SPI-1. The lineages are arranged according to the size of the deletion they incurred. The deleted regions are marked with a dashed line. The grey box encompasses a region common to all five deletions that encoded the prg/org locus and the promoter and start codon of hilD. Lineage C did not acquire a deletion or any other mutations in the SPI-1 region.