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. 2014 Nov 6;10(11):e1004500. doi: 10.1371/journal.ppat.1004500

Figure 4. Disruption of SPI-1 expression improves fitness of an hns mutant.

Figure 4

(A) Growth of a wild type S. Typhimurium (black curve) and a Δhns strain (green curve) was monitored in liquid media alongside wild type and Δhns strains harboring a 40 kb SPI-1 deletion (blue and red curves respectively). Elimination of the SPI-1 locus significantly increased growth of the Δhns strain and also provided a slight growth advantage in the wild type background. (B) Targeted disruption of hilD, the master SPI-1 activator produces a similar growth outcome as the SPI-1 deletion. Growth curves of the ΔhilD and ΔhilD/Δhns strains are represented in orange and purple respectively. The corresponding wild type (black) and hns mutant (green) growth curves are the same as panel A. Plotted is the average of three biological replicates and standard error (panels A and B).