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. 2014 Nov 6;10(11):e1004487. doi: 10.1371/journal.ppat.1004487

Figure 6. Loss of srbB impairs heme biosynthesis and results in accumulation of heme intermediates.

Figure 6

(A–B). Amount of Protoporphyrin IX (PP IX) and intermediate compounds including uroporphyrin (C8), heptacarboxylporphyrin (C7), hexacarboxylporphyrin (C6), pentacarboxylporphyrin (C5), and coproporphyrin (C4) were analyzed using HPLC. Mycelia used for HPLC analysis were harvested from cultures in LGMM at 37°C for 2 days in hypoxia. Compared to wild type, ΔsrbB produces more PP IX and other intermediates in hypoxia. (A) is a chromatogram from HPLC analysis, and (B) is a graph to present the HPLC result with statistical analysis. Data are presented as the mean and standard error of three biological replicates, and analyzed by one-way ANOVA followed by a Tukey's multiple comparison test. (C). A thousand conidia were inoculated on GMM or GMM containing 5 µM hemin. In 2 days, radial growth of each strain in normoxia or hypoxia was observed. Addition of hemin improved ΔsrbB growth in hypoxia.