Fig. 1.

Time course of fasting-induced liver TG accumulation in ICR mice. First we validated the effects of fasting periods on the accumulation levels of hepatic TG in 8-week-old female ICR mice, because the TG accumulations to liver may be different at a strain, sex, and week old of mice. At the 6-h, 12-h, 18-h 24-h, 30-h, 36-h, or 48-h after the fasting respectively, the mice were sacrificed and collected a piece of liver tissues. The extract from each animal liver tissue was measured by an enzymatic colorimetric method. Values shown are the means ± S.E.M. of 4–11 mice. ^∗Significant difference from ad libitum-fed mice (one-way ANOVA, Dunnett’s post hoc test, P < 0.05).