Fig. 5.

Effect of AChE inhibitor physostigmine on the gene expression of L-Fabp (A), Srebp1c (B), Acc (C), Pparα (D), Pgc1α (E), Cpt1 (F) Pepck (G), and Irs2 (H) in liver extracts from the 30-h fasted mice. Mice underwent a 30-h fast from ZT6 to ZT36, and given twice a day subcutaneous (s.c.) injection of 0.3 mg/kg physostigmine or saline at ZT24 and ZT30. Muscarinic receptor antagonist atropine (1 mg/kg, s.c.) was administered 15 min before the physostigmine injections. At the 30-h after the fasting, the mice were sacrificed and collected a piece of liver tissues. Total RNA was extracted from a piece of the liver tissues and performed PCR as described in Section 2. The relative levels of the target gene PCR product were normalized to those of 18srRNA. Values shown are the means ± S.E.M. of 4–6 mice. ^∗Significant difference from 30 h-fasted mice with saline (described 0 mg/kg physostigmine in figure) (t-test or Wilcoxon–Mann–Whitney test, based on the F value, P < 0.05).