Fig. 8.

Effect of AChE inhibitor physostigmine on plasma glucagon (A), plasma LDL/VLDL (B), and plasma FFA (C) in fasted and fed mice. Mice underwent a 30-h fast from ZT6 to ZT36, and given twice a day subcutaneous (s.c.) injection of 0.3 mg/kg physostigmine or saline at ZT24 and ZT30. Muscarinic receptor antagonist atropine (1 mg/kg, s.c.) were administered 15 min before the physostigmine injections. To examine the effect of fasting duration, we also measured the plasma FFA concentration in 24 h-fasted mice. At the 30-h or 24-h after the fasting, the mice were sacrificed and collected whole blood. The plasma specimen measured by the fluorometric or colorimetric enzyme assay kit for glucagon, LDL/VLDL, or FFA, respectively. Values shown are the means ± S.E.M. of 6–16 mice. ^∗Significant difference from ad lib-fed mice (t-test, P < 0.05). ^#Significant difference between two groups (t-test, P < 0.05).