Figure 3.

Binding of thiophosphorylated hTH1–50 peptides to 14-3-3ζ. (A) ³¹P spectra of tpS40-hTH1–50 peptide (0.71 mM) for varying molar ratios of 14-3-3ζ to the peptide (r); 0:1 (blue), 0.5:1 (red), 1:1 (dark green), 2:1 (purple), 4:1 (orange), and 8:1 (brown). (Inset) The chemical shift changes upon 14-3-3ζ binding (Δδ) are plotted as a function of molar ratio (r). (Continuous line) Predicted Δδ from fitting the experimental Δδ to the quadratic equation shown in Eq. 2. (B) ³¹P spectra of pS19tpS40-hTH1–50 (0.83 mM) for varying molar ratios of 14-3-3ζ to the peptide (r): 0:1 (blue), 0.5:1 (red), 1:1 (dark green), 2:1 (purple), 3:1 (yellow), 4:1 (orange), and 5:1 (light green). To see this figure in color, go online.