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. 2014 Nov 4;107(9):2130–2140. doi: 10.1016/j.bpj.2014.09.038

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© 2014 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).

PMC Copyright notice

The local interactions that stabilize Tyr⁶³⁹ in the active site of the preinsertion complex with different nucleotides bound. The histograms are sampled from 10 to 100 ns in each individual simulation. (a) The interactions between Tyr⁶³⁹ and the end bp of the DNA-RNA hybrid. (b) The interactions between Tyr⁶³⁹ and the preinserted nucleotide, rATP, dATP, rGTP, rUTP, and rCTP.