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. 2014 Sep 18;289(45):31001–31013. doi: 10.1074/jbc.M114.580811

FIGURE 2.

FIGURE 2.

Kindlin-2 interacts with Src in a FAK-regulatable fashion. A and B, GST pull-down experiments were performed using lysates from human podocytes (A) or from FAK-positive (FAK+/+) or knock-out (FAK−/−) mouse embryonic fibroblasts (B) as described under “Experimental Procedures.” The cell lysates (A, lane 1; B, lanes 1 and 5), GST pull-downs (A, lane 4; B, lanes 2 and 6), and GST-kindlin-2 pull-downs (A, lane 3; B, lanes 3 and 7) were analyzed by Western blotting with antibodies recognizing FAK, Src, or ILK or Coomassie Blue staining. The samples in lane 2 of A and lane 4 of B were prepared as those in lane 3 of A and lane 3 of B except that cell lysates were omitted. C, cell lysates (lanes 1 and 2), anti-kindlin-2 immunoprecipitates (lanes 5 and 6), or control IgG immunoprecipitates (lanes 3 and 4) derived from FAK knock-out cells (lanes 2, 4, and 6) or FAK-positive cells (lanes 1, 3, and 5) were analyzed by Western blotting with antibodies recognizing FAK, kindlin-2, or Src.