FIGURE 7.

Identification of RIPK4-dependent regulatory phosphorylation sites in IRF6. A, schematic of IRF6 in which the positions of Ser-413 and Ser-424 are indicated. DBD, DNA-binding domain; IAD, IRF association domain. B and C, HEK293T cells were transfected with an IFNβ gene promoter reporter plasmid together with expression vectors encoding the indicated proteins. Gene reporter activity was measured 24 h post-transfection, and IRF6 activity is shown as the fold increase. *, p < 0.05; **, p < 0.01. The data are combined from three independent experiments.