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Summary of substrate and filter setting used in BRET assays
| Method | Donor | Substrate | Donor emission | Acceptor | Acceptor emission |
|---|---|---|---|---|---|
| nm | nm | ||||
| BRET1 | Rluc | Coelenterazine h | 460 | EGFP | 535 |
| BRET2 | Rluc | Deep Blue C | 400 | GFP2 | 515 |
| eBRET | Rluc | EnduRenTM | 460 | EGFP | 535 |
