TABLE 2.
Kinetic parameters for ATP hydrolysis determined by a biochemical assay of EhVoV1 and EhV1
| Protein | pH | NaCl | KmATP | VmaxATP | konATP (3 × VmaxATP/KmATP)a | Reference |
|---|---|---|---|---|---|---|
| mm | μm | rps | m−1 s−1 | |||
| Native EhVoV1 | 8.5 | 300 | 86 ± 7 | 40 ± 0.8 | (1.4 ± 0.1) × 106 | This study |
| Recombinant EhVoV1 | 8.5 | 300 | 65 ± 2 | 37 ± 0.2 | (1.7 ± 0.1) × 106 | This study |
| Recombinant EhVoV1 | 6.5 | 300 | 134 ± 12 | 59 ± 1.4 | (1.3 ± 0.1) × 106 | This study |
| Recombinant EhV1 | 6.5 | 0 (50 mm KCl) | 221 ± 17 | 73 ± 1.5 | (1.0 ± 0.1) × 106 | Ref. 29 |
a The second order binding rate constant for ATP (konATP) determined from 3 × VmaxATP/KmATP.