TABLE 2.
Yeast strains used in this study
| Strain | Genotype | Source |
|---|---|---|
| W303-1A | MAT a; ura3-1; trp1-1; leu2–3_112; his3-11; ade2-1; can1-100 | EUROSCARF |
| BY4741 | Mat a; his3D1; leu2D0; met15D0; ura3D0 | EUROSCARF |
| Δatg1 | BY4741; Mat a; his3D1; leu2D0; met15D0; ura3D0; YGL180W::kanMX4 | EUROSCARF |
| Δatg7 | BY4741; Mat a; his3D1; leu2D0; met15D0; ura3D0; YHR171W::kanMX4 | EUROSCARF |
| smt3ts | S542: MAT α, smt3-331 | 48 |
| RH3601 | smt3ts containing two genomic copies GAL1::SNCAWT::GFP in URA3 locus | This study |
| RH3602 | W303 containing two genomic copies GAL1::SNCAK96R/K102R::GFP in URA3 locus | This study |
| ulp1ts | 82 | |
| RH3603 | ulp1ts containing YIplac211-ADH-His6-Smt3 in HIS3 locus | This study |
| RH3604 | RH3603 containing GAL1::SNCAWT integrated in TRP1 locus | This study |
| RH3605 | RH3603 containing GAL1::SNCAA30P integrated in TRP1 locus | This study |
| RH3606 | RH3603 containing GAL1::SNCAK96R/K102R integrated in TRP1 locus | This study |
| RH3607 | smt3ts containing 2 genomic copies GAL1::SNCAS129A::GFP in TRP1 locus | This study |