TABLE 1.
Analysis of gene expression in NSPCs from APPKO using a mouse neurogenesis and neural stem cell PCR array
Mouse PCR neurogenesis array results show the expression of genes in APPKO NSPCs that are significantly up-regulated or down-regulated relative to NSPCs from the corresponding background strain WT mice. RNA was extracted from NSPC cultures derived from three independent mouse cohorts. For the data analysis, the ΔΔCt method was used (64). For a -fold change of >1, the result is reported as a -fold up-regulation. For a -fold change <1, the negative inverse of the result is shown as a -fold down-regulation. Values of -fold up- or down-regulation >2 or <−2 were assumed to be significant.
| Gene name | Expression level (relative to β-actin) |
-Fold up- or down- regulation in KO | |
|---|---|---|---|
| APPKO | WT | ||
| Amyloid-β (A4) precursor protein | 1.2 × 10−6 | 2.2 × 10−3 | −1803.60 |
| Bone morphogenetic protein 8b | 1.8 × 10−6 | 6.8 × 10−7 | 2.60 |
| Fibroblast growth factor 2 | 2.2 × 10−5 | 5.1 × 10−5 | −2.35 |
| Glial cell line-derived neurotrophic factor | 3.5 × 10−6 | 8.9 × 10−6 | −2.57 |
| Hairy/enhancer-of-split related with YRPW motif1 | 3.0 × 10−4 | 6.7 × 10−4 | −2.26 |
| Hairy/enhancer-of-split related with YRPW motif2 | 5.2 × 10−6 | 1.2 × 10−5 | −2.38 |
| Midkine | 1.2 × 10−4 | 3.4 × 10−5 | 3.37 |
| Neurogenin 2 | 3.5 × 10−7 | 1.4 × 10−5 | −41.69 |
| Neurofibromatosis 1 | 9.3 × 10−4 | 2.3 × 10−4 | 4.02 |
| Noggin | 1.2 × 10−5 | 3.3 × 10−5 | −2.69 |
| Neuropilin 1 | 2.3 × 10−4 | 8.9 × 10−5 | 2.57 |
| Odd Oz/ten-m homolog 1 | 2.3 × 10−6 | 6.5 × 10−7 | 3.50 |
| Paired box gene 3 | 3.0 × 10−6 | 1.6 × 10−5 | −5.33 |
| Paired box gene 5 | 2.6 × 10−3 | 7.4 × 10−3 | −2.83 |
| POU domain, class 3, transcription factor 3 | 2.2 × 10−3 | 1.9 × 10−2 | −8.79 |
| Sonic hedgehog | 3.9 × 10−5 | 1.8 × 10−5 | 2.14 |
| Tyrosine hydroxylase | 2.0 × 10−6 | 5.9 × 10−7 | 3.44 |