FIGURE 10.

Effects of depletion of K-Ras, RalA, and RalB on anchorage-independent growth. H460 or HCT116 cells were transfected with indicated siRNAs for 48 h before plating in soft agar as described under “Experimental Procedures.” Results are shown as average percent inhibition of four independent experiments (H460) or three independent experiments (HCT116). Nontargeting (NT) and nontargeting + p53 siRNAs were set as 100%. For H460, mean number of colonies for nontargeting was 323 ± 8.5 and for nontargeting + p53 siRNA was 232 ± 66.22. For HCT116, mean number of colonies for nontargeting was 469 ± 7.2 and for nontargeting + p53 siRNA was 484 ± 6. Error bars show S.E. Student's t test was used to compare the means; p values are shown for tests that show significant differences (*, p < 0.05). Top panel shows representative soft agar pictures for HCT116 cells.