FIGURE 11.

Effects of depletion of K-Ras, RalA, and RalB on invasion. A549, H460, or HCT116 cells were transfected with the indicated siRNAs for 48 h, and then equal numbers of cells were seeded for invasion assays as described under “Experimental Procedures.” The cells were then photographed and counted to determine the mean number of invading cells. Top panel shows representative image of invading cells of H460, and bottom panels show average number of invading cells. p values for H460 are K-Ras = 0.02, RalA = 0.02, and RalB = 0.03. p values for HCT116 are K-Ras = 0.006 and RalB = 0.007. p values for A549 are K-Ras = 0.004 and RalB = 0.0006. NT, nontargeting.