FIGURE 6.

Ang-2 does not compete with fibronectin in binding to α5β1 integrin. A, a schematic diagram of integrin domain structure. The integrin domain structure was adapted and modified from Zhu et al. (27). B, biotinylated fibronectin (5 μg/ml) was incubated in the presence of increasing amounts of Ang-2 on the plates coated with α5β1 integrin (5 μg/ml). Bound fibronectin was measured in a colorimetric reaction detected by streptavidin conjugated with HRP and TMB. C, biotinylated fibronectin was incubated on a α5β1 integrin-coated plate in the presence of 20 μg/ml integrin blocking antibodies, 20 μg/ml Ang2, or 10 mm EDTA. Bound fibronectin was measured as described in B.