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. 2014 Sep 18;289(45):31341–31348. doi: 10.1074/jbc.M114.605451

FIGURE 3.

FIGURE 3.

Structure of the Pt-GG·dCTP*-Mg2+ ternary complex (Protein Data Bank code 4TUR). A, close-up view of the Pt-GG lesion in the polβ:Pt-GG-Mg2+ complex. Platinum and NH3 are shown in gray and blue spheres, respectively. B, a 2Fo − Fc electron density map contoured at 1σ around the primer terminus and the replicating base pairs of the Pt-GG lesion. C, close-up view of the active site of the Pt-GG-Mg2+ complex. The three catalytic aspartates (Asp-190, Asp-192, and Asp-256) and the minor groove-interacting amino acids (Tyr-271, Asn-279, and Arg-283) are indicated. D, overlay of the active sites of the Pt-GG·dCTP*-Mg2+ (multicolored) and the unmodified GG·dCTP*-Mg2+ (white) ternary structures. E, overlay of the overall structures of the Pt-GG·dCTP*-Mg2+ complex (multicolored; Protein Data Bank code 4TUR) and the unmodified GG·dCTP*-Mg2+ complex (white; Protein Data Bank code 4TUQ).