TABLE 2.

Steady-state kinetic parameters for dCTP insertion opposite the templating dG by polβ

Template·dCTP	Metal ion	Km	kcat	kcat/Km	f a
		μm	10−3 s−1	10−3 s−1 μm−1
dG·dCTP	Mg2+	0.67 ± 0.03	164.30 ± 6.65	245.2	1
Pt-GG·dCTP	Mg2+	5.22 ± 1.01	15.76 ± 1.24	3.0	1.2 × 10−2
Pt-GG·dCTP	Mn2+	1.14 ± 0.05	27.60 ± 1.62	24.2	9.9 × 10−2

^a f = (k_cat/K_m)_{[Pt-GG·dCTP]}/(k_cat/K_m)_(dG·dCTP).