FIGURE 8.

NGF does not affect the rate of nuclear import of NFATc3 in DRG neurons. A, nuclear transport of EGFP-NFATc3 (green) was simultaneously monitored with [Ca²⁺]_i changes (black) in DRG neurons as described previously (7, 33) (also see “Experimental Procedures”). DRG cultures were deprived of NGF for 24 h before the experiment was initiated. [Ca²⁺]_i elevations and nuclear import of EGFP-NFATc3 were induced by mild depolarization with K⁺10 (supplemented with 1 μm BayK8644). The nuclear import of EGFP-NFATc3 was quantified by measuring EGFP fluorescence intensity in the nuclear region and is expressed as the average rate (slope) of translocation into the nucleus during the first 5 min of translocation (dotted rectangular box). B, superimposition of representative traces showing EGFP-NFATc3 nuclear import in the absence (green) or presence (blue) of 25 ng/ml NGF or in neurons transfected with GSK3β shRNA (red). C, quantification of the rate of EGFP-NFATc3 nuclear import in experiments like those described in A and B. No significant differences were observed under the conditions described (n = 4–10 cells). a.u., arbitrary units. Error bars, S.E.