FIGURE 9.

A combination of curcumin (Cur) with either gA or ouabain (Oua) stimulates selective cell death of HEK-ABCG2 cells over parental cells. Cells were incubated in the absence (A) or presence of various compounds: 35 nm gA (B), a combination of 35 nm gA and 2 μm curcumin (C), 7.5 nm ouabain (D), and a combination of 7.5 nm ouabain and 2 μm curcumin (E) for 24, 48, and 72 h and were subsequently stained with annexin V-FITC/EthD I for analysis using a flow cytometer. The dark gray bars indicate the population of early apoptotic cells (annexin V-FITC +/EthD I −), whereas the light gray bars indicate the population of late apoptotic or necrotic cells (annexin V-FITC +/EthD I +).