TABLE 3.

IC₅₀ values, relative resistance, and reversal of resistance of CCCP and 2-DG in HEK-293 parental cells and in HEK-ABCG2 cells

Compound	IC50		p value of IC50 from HEK-ABCG2 cells relative to IC50 from control cellsa	Relative resistanceb	Reversal of resistancec
	HEK-293 control	HEK- ABCG2
CCCP alone	2.3 ± 0.4 μm	2.4 ± 0.3 μm	0.32	1.0 ± 0.2-fold
CCCP + 2 μm curcumin	1.8 ± 0.2 μm	1.7 ± 0.2 μm	0.16	0.9 ± 0.2-fold	1.1 ± 0.3-fold
2-DG alone	4.0 ± 0.6 mm	4.2 ± 0.9 mm	0.27	1.0 ± 0.3-fold
2-DG + 2 μm curcumin	2.1 ± 0.3 mm	2.0 ± 0.4 mm	0.18	1.0 ± 0.2-fold	1.0 ± 0.4-fold

^a To determine p values, we used a two-tailed Student's t test based on log(IC₅₀) values. We considered p values ≤ 0.05 as statistically significant.

^b Expressed as the ratio of the IC₅₀ value of ABCG2-expressing cells to that of the control cells.

^c Expressed as the ratio of the relative resistance value of ABCG2-expressing cells in the absence of the second compound to the relative resistance in its presence.