TABLE 1.

Comparison of two-component fits to α4β2 concentration-response relations in N2a cells when co-transfected with empty vector, or one of three lynx1 conditions

lynx1, mCherry-lynx1, or lynx1 plus PLC treatment was used. Fits were generated under two assumptions: 1) all HS receptors, and no LS receptors, are activated at 10 μm ACh, and 2) Hill coefficients were constrained to ≤1.5. Fitted parameters for ACh concentration-response relations of α4β2 when co-transfected with empty vector, lynx1-wt, mCherry-lynx1, and lynx1-wt after treatment with PI-PLC. Parameters correspond to ACh concentration-response relations in Fig. 3. The EC₅₀ and Hill coefficient values represent the means ± S.E. for the number of measurements (n) obtained. The relative fraction of the high sensitivity component (first component) for the biphasic relations is included.

α4β2 ACh	HS				LS				HS	R2
	EC50	EC50 error	n	n error	EC50	EC50 error	n	n error
									%
WT +pcDNA	2.34	1.38	0.86	0.43	36.9	20.1	1.5	0.76	50.3	0.97
+lynx-pTracer	5.42	3.36	1.5	1.18	61.4	14.1	1.5	0.44	27.0	0.99
+ mC-lynx	2.49	1.41	1.05	0.76	58.6	12.8	1.5	0.37	28.4	0.99
Lynx+PLC	6.55	9.47	0.54	0.41	41.9	14.7	1.5	0.37	24.9	0.99