FIGURE 1.

miR-141 and miR-146b-5p repress the expression of AUF1. A, sequence alignment of human miR-141 and miR-146b-5p binding sites in the AUF1 3′-UTR. B, the binding sites of miR-141 and miR-146b-5p in the AUF1 3′-UTR in different species. C and E, total RNA was purified from the indicated cells harboring the indicated constructs, and the indicated RNAs were amplified by qRT-PCR using specific primers. Error bars, S.E. values of three different experiments; *, p ≤ 0.001. D, total RNA was prepared from the indicated cells and was used for Northern blot analysis.