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. 2014 Sep 26;289(45):31433–31447. doi: 10.1074/jbc.M114.593004

FIGURE 2.

FIGURE 2.

miR-141 and miR-146b-5p negatively regulate the expression of the AUF1 mRNA via its 3′-UTR. Cells were transfected with miRZip-141 (A) and miRZip-146b-5p (B), and then total RNA was purified from the indicated cells and amplified by qRT-PCR. Error bars, S.E. values of three different experiments. *, p < 0.0002. C, schematic representation of the luciferase reporter vector bearing the AUF1 3′-UTR. D, sequence alignment of human miR-141 and miR-146b-5p binding sites in the AUF1 3′-UTR showing the mutated sequences (boldface type). E, U2OS cells expressing pre-miR-141, pre-miR-146b-5p, or a control plasmid were stably transfected with the luciferase reporter vector bearing either the wild type AUF1 3′-UTR or a mutated sequence for one of the binding sites of miR-141 (residues 297–304) or miR-146b-5p. The reporter activity was assessed at 48 h post-transfection. Data (mean ± S.E., n = 4) are presented as percentage change in reporter activity as compared with the negative control cells (*) or to the wild type 3′-UTR (**). * and **, p < 0.0004.