FIGURE 3.

miR-141 and miR-146b-5p repress the expression of the AUF1 protein. A and B, whole protein extracts were prepared and used for immunoblotting analysis utilizing antibodies against the indicated proteins. The numbers below the bands indicate the corresponding expression levels after loading correction against GAPDH. C, whole cell lysates were prepared from U2OS and EH1 expressing the indicated constructs, and 400 μg of proteins were used for immunoprecipitation with anti-AUF1 antibody or anti-IgG (Control). The level of the CDKN1A mRNA was assessed by qRT-PCR of the corresponding immunoprecipitation material using specific primers. Error bars, S.E. values of three different experiments. D and E, total RNA was purified from the indicated cells, and the CDKN1A mRNA was amplified by qRT-PCR using specific primers. Error bars, S.E. values of three different experiments; *, p ≤ 0.001.