FIGURE 5.

miR-141 and miR-146b-5p repress the invasion/migration potential of U2OS cells through inhibition of AUF1. A and B, U2OS cells were transfected with the indicated constructs, and cells (2 × 10⁴) were added in serum-free medium to the upper wells of the CIM plates (Roche Applied Science) separated by an 8-μm pore size PET membrane with a thin layer of Matrigel basement membrane matrix (Invasion) or without (Migration), and the migration/invasion were assessed for 24 h using the RTCA instrument. Each assay was performed in triplicate. The results were expressed as mean cells/well. C, cells were transfected with the indicated constructs, and the proliferation rate was measured several passages post-transfection using the RTCA instrument and E-plates.