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. 2014 Sep 24;289(45):31458–31472. doi: 10.1074/jbc.M114.589937

FIGURE 5.

FIGURE 5.

Reduction in RacGTP levels in the cerebellum of p75 knock-out mice. A, RacGTP is enriched in the synaptosome fraction. Proteins in synaptosome (Syn) and S2 fractions were subjected to GST-PAK1 pull-down to detect GTP-bound Rac1 prior to Rac1 Western blotting. Note that the majority of Rac1 localized to the synaptosome fraction is GTP-bound. In contrast, Cdc42GTP is predominantly localized to the S2 fraction. PSD95 is a control for the fractionation. Cb, cerebellum; Cx, cortex. B, RacGTP levels and the levels of its downstream effector, p-PAK, are significantly reduced in the cerebellum of p75−/− mice compared with the wild type. Note that the reduction is restricted to the cerebellum and not found in the cortex of the same animal. C, Quantification of RacGTP levels between p75+/+ and p75−/− mice. D, quantification of p-PAK levels between p75+/+ and p75−/− mice. E, images of p-PAK immunohistochemistry in the molecular layer of the cerebellum in p75+/+ and p75−/− mice.