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. 2014 Sep 24;289(45):31458–31472. doi: 10.1074/jbc.M114.589937

FIGURE 8.

FIGURE 8.

Reduction in the number of spines in Purkinje cells in the absence of p75. A, representative images of Purkinje cells stained with Golgi from adult p75+/+ and p75−/− mice. Scale bar, 25 μm. B, representative images of Purkinje cell dendrites that were used for quantification of spines. Scale bar, 6 μm. C, quantification of the number of spines. Five Purkinje neurons in lobules 4, 5, 6, and 7 per mouse were analyzed using the Neurolucida® version 7 image analysis system (MBF Biosciences), and the spine density was estimated (n = 3 mice). For each Purkinje neuron, at least five different secondary and tertiary dendritic branches were randomly selected for spine analysis at ×100 magnification to obtain the estimated values for the number of spines in a given length. D, representative EM images of Purkinje cell spines from adult p75+/+ and p75−/− mice. E, quantification of spine area and spine length based on EM images collected from three different sets of mice and a total of 29 spines with features of post-synaptic density (PSD) using ImageJ.