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BMP4-induced pSmad1/5/8 nuclear translocation is Smad4-independent in dental mesenchymal cells. Primary dental mesenchymal cells from R13.5 Smad4f/f mouse embryo were infected with Cre-lentivirus. A and B, knock-out efficiency of Smad4 was confirmed by immunofluorescence (A) and Western blot (B). C, immunofluorescence shows nuclear translocation of pSmad1/5/8 in cells lacking Smad4 after BMP4 stimulation for 30 min.
