Sequence alignment of the repeats in PPR10.
A, a sequence logo generated using repeat sequences of PPR10 reveals relatively conserved amino acid positions within the repeats. The amino acids in the repeats are colored based on the physiochemical properties of the side chains: small (Gly (G) and Ala (A)) in black, nucleophilic (Ser (S), Thr (T), and Cys (C)) in blue, hydrophobic (Val (V), Leu (L), Ile (I), Met (M), and Pro (P)) in green, aromatic (Phe (F), Tyr (Y), and Trp (W)) in red, acidic (Asp (D) and Glu (G)) in light pink, amide (Asn (N) and Gln (Q)) in dark pink, and basic (His (H), Lys (K), and Arg (R)) in orange (33, 44). B, the principal portion of PPR10 repeats (residues 107–771) is summarized in the table of 19 repeat alignment. The molecular determining residues for RNA recognition at the 2nd, 5th, and 35th positions are shaded light gray.