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. 2014 Sep 17;289(45):31503–31512. doi: 10.1074/jbc.M114.575472

FIGURE 3.

FIGURE 3.

Binding affinity assays of different amino-terminal truncations of PPR10 with RNA. A, EMSAs are performed for different amino-terminal PPR10 with RNA elements. The RNA sequences of psaJ and atpH are indicated in Fig. 1B. Binding reactions contain ∼40 pm radiolabeled RNAs and PPR10 proteins at the indicated concentrations. B, the PPR10 fractions of RNA bound and concentrations in each lane of Fig. 2B are plotted and fitted to the calculate equilibrium Kd values. C, the summary table of equilibrium Kd values for EMSA reactions of different amino-terminal PPR10 with psaJ and atpH RNA elements.