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. 2014 Sep 24;289(45):31526–31533. doi: 10.1074/jbc.M114.584565

FIGURE 1.

FIGURE 1.

Sfrp1 increases after unilateral ureteral obstruction (UUO). A, the Sfrp1 antibody reacted specifically with a band corresponding to Sfrp1 in lysates of Sfrp1-FLAG-expressed 293T cells, but not 293T (mock) cells by Western blotting. B, characterization of an antibody specifically recognized Sfrp1, Sfrp2, and Sfrp5. Immunoreactivity was impaired specifically with Sfrp1, but not with other Sfrp protein families (Sfrp2–5). The Sfrp2 and Sfrp5 antibodies were also immunoreacted specifically with Sfrp2 and Sfrp5 proteins, respectively. C, the levels of the Sfrp1, Sfrp2, and Sfrp5 proteins were detected by Western blot analysis in the Sham and UUO kidneys in wild-type mice (Sfrp1+/+) or Sfrp1 knock-out mice (Sfrp1−/−) at different time points after UUO. Actin was evaluated as an internal control. His-Sfrp2 and His-Sfrp5 were used as a positive control to detect Sfrp2 and Sfrp5 proteins, respectively.