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. 2014 Sep 24;289(45):31526–31533. doi: 10.1074/jbc.M114.584565

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Wnt/β-catenin pathway in the Sfrp deficient obstructed kidneys. A, detection of active- and total β-catenin, c-Myc, and cyclin D1 by Western blotting of the UUO kidneys. Actin was evaluated as an internal control. B, immunohistochemical analyses of mouse kidney sections with antibodies against active and total β-catenin in the sham and UUO kidneys at 7 days after the surgery. C and D, number of phospho-HistonH3 (Ser-10) cells in the obstructed kidneys at 7 days after UUO. C, section of the Sham and UUO kidneys in Sfrp^+/+ and Sfrp^−/− mice were subjected to immunostaining with an anti-phospho-histone H3 (Ser-10) antibody. D, graph shows analysis of the percentage of phospho-histone H3 (Ser-10)-positive cells in the UUO kidneys. Scale bars, 100 μm.