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. 2014 Sep 24;289(45):31526–31533. doi: 10.1074/jbc.M114.584565

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Non-canonical Wnt pathway in the Sfrp-deficient obstructed kidneys. A, detection of phospho- and total c-Jun, phospho- and total JNK, phospho- and total p38, and phospho- and total Ca²⁺/calmodulin-dependent kinase II (CaMKII) by Western blotting of the UUO kidneys. Actin was evaluated as an internal control. B, immunohistochemical analyses of mouse kidney sections with antibodies against phospho- and total c-Jun in the sham and UUO kidneys at 7 days after the surgery. C and D, number of TUNEL-positive cells in the obstructed kidneys at 7 days after UUO. C, section of the sham and UUO kidneys in Sfrp^+/+ and Sfrp^−/− mice were subjected to TUNEL staining. D, graph shows analysis of the percentage of TUNEL-positive cells in the UUO kidneys. Scale bars, 100 μm. *, p < 0.05.