FIGURE 2.

Generation of Apc^min/+ mice with different GnT-V genotypes. A, normal and neoplastic crypts of Apc^min/+ mice were immunostained with L-PHA. Arrows indicate normal and early neoplastic crypts. Bar, 20 μm. B, after genomic DNA was extracted from tails of Apc^min/+ mice, genotyping was performed to detect the expression of mutated Apc, wild-type GnT-V, and LacZ genes by PCR. C, adenoma sections from different GnT-V genotypes using 18-week-old adenoma tissues prepared and stained with hematoxylin/eosin and L-PHA. Bar, 50 μm.