TABLE 1.
Gene | Forward primer (5′-3′) | Reverse primer (5′-3′) |
---|---|---|
Human | ||
MGAT5 | gagcagatcctggacctcag | gctgtcatgactccagcgta |
GAPDHa | tggacctgacctgccgtctag | cctccgacgcctgcttcac |
CCND1 | aactacctggaccgcttcct | ccacttgagcttgttcacca |
CMYC | ttcgggtagtggaaaaccag | cagcagctcgaatttcttcc |
LGR5 | ctcttcctcaaaccgtctgc | gatcggaggctaagcaactg |
ASCL2 | gtgaagctggtgaacttgggc | cagcgtctccaccttgctca |
AXIN2 | ctccttatcgtgtgggcagt | cttcatcctctcggatctgc |
APCDD1 | catccagacagcaggtctca | gggcctgaccttacttcaca |
DKK1 | gccccgggaattactgcaaaaatg | ccggagacaaacagaaccttcttgt |
ALDH1 | ttggaatttcccgttggtta | ctgtaggcccataaccagga |
CD133 | agtggcatcgtgcaaacctg | ctccgaatccattcgacgatagta |
BMI1 | gagggtacttcattgatgccacaac | gctggtctccaggtaacgaacaata |
SOX2 | tgagcgccctgcagtacaa | gctgcgagtaggacatgctgtag |
OCT4 | ctggagaaggagaagctgga | caaattgctcgagttctttctg |
Mouse | ||
Mgat5 | ccctggaagttgtcctctca | tcctctgccagtgccttaat |
Gapdha | tgcgacttcaacagcaactc | atgtaggccatgaggtccac |
Cmyc | ctagtgctgcatgaggagacac | gtagttgtgctggtgagtggag |
Lgr5 | ccttggccctgaacaaaata | atttctttcccagggagtgg |
Ascl2 | caggagctgcttgacttttcca | gggctagaagcaggtaggtcca |
Axin2 | gcagctcagcaaaaagggaaat | tacatggggagcactgtctcgt |
Keratin20 | gcacagattaaagagctgcaaa | cactgggcattttgtacttgag |
a Normalization control gene.