. 2014 Oct 1;289(45):31534–31549. doi: 10.1074/jbc.M114.602680

TABLE 1.

Sequences of primers used in qRT-PCR

Gene	Forward primer (5′-3′)	Reverse primer (5′-3′)
Human
MGAT5	`gagcagatcctggacctcag`	`gctgtcatgactccagcgta`
GAPDH^a	`tggacctgacctgccgtctag`	`cctccgacgcctgcttcac`
CCND1	`aactacctggaccgcttcct`	`ccacttgagcttgttcacca`
CMYC	`ttcgggtagtggaaaaccag`	`cagcagctcgaatttcttcc`
LGR5	`ctcttcctcaaaccgtctgc`	`gatcggaggctaagcaactg`
ASCL2	`gtgaagctggtgaacttgggc`	`cagcgtctccaccttgctca`
AXIN2	`ctccttatcgtgtgggcagt`	`cttcatcctctcggatctgc`
APCDD1	`catccagacagcaggtctca`	`gggcctgaccttacttcaca`
DKK1	`gccccgggaattactgcaaaaatg`	`ccggagacaaacagaaccttcttgt`
ALDH1	`ttggaatttcccgttggtta`	`ctgtaggcccataaccagga`
CD133	`agtggcatcgtgcaaacctg`	`ctccgaatccattcgacgatagta`
BMI1	`gagggtacttcattgatgccacaac`	`gctggtctccaggtaacgaacaata`
SOX2	`tgagcgccctgcagtacaa`	`gctgcgagtaggacatgctgtag`
OCT4	`ctggagaaggagaagctgga`	`caaattgctcgagttctttctg`

Mouse
Mgat5	`ccctggaagttgtcctctca`	`tcctctgccagtgccttaat`
Gapdh^a	`tgcgacttcaacagcaactc`	`atgtaggccatgaggtccac`
Cmyc	`ctagtgctgcatgaggagacac`	`gtagttgtgctggtgagtggag`
Lgr5	`ccttggccctgaacaaaata`	`atttctttcccagggagtgg`
Ascl2	`caggagctgcttgacttttcca`	`gggctagaagcaggtaggtcca`
Axin2	`gcagctcagcaaaaagggaaat`	`tacatggggagcactgtctcgt`
Keratin20	`gcacagattaaagagctgcaaa`	`cactgggcattttgtacttgag`

^a Normalization control gene.