FIGURE 7.

Glycosylations that are involved in intermolecular interactions. A, three asymmetric units of Pp-HjCel3A, each containing two protein molecules, are shown in blue, purple, and green. The crystal contacts with van der Waals interaction between glycan molecules are indicated by black circles. The intermolecular interactions involve both of the glycosylation sites at Asn²⁰⁸, located at the dimer interface of the non-crystallographic symmetry-related molecules, and at Asn³¹⁰. B, interaction of the NAG molecule at Asn³¹⁰ at one of the crystal contacts in the HjCel3A structure. This NAG glycan makes both direct interaction and water-mediated interactions with residues of a neighboring molecule.