Table 2.
Primers used for RT-PCR analysis
| Target gene | Primer sequence | Annealing temp |
|---|---|---|
| TNF-α |
F: 5′-AGCACAGAAAGCATGATCCG-3′ |
55°C |
| |
R: 5′-GTTTGCTACGACGTGGGCTA-3′ |
|
| IL-6 |
F: 5′-CATGTTCTCTGGGAAATCGTGG-3′ |
58°C |
| |
R: 5′-AACGCACTAGGTTTGCCGAGTA-3′ |
|
| IL-1β |
F: 5′-TGCAGAGTTCCCCAACTGGTACATC-3′ |
64°C |
| |
R: 5′-GTGCTGCCTAATGTCCCCTTGAATC-3′ |
|
| COX-2 |
F: 5′-CACTCAGTTTGTTGAGTCATTC-3′ |
45°C |
| |
R: 5′-GATTAGTACTGTAGGGTTAATG-3′ |
|
| iNOS |
F: 5′-AGCCCAACAATACAAATGACCCTA-3′ |
56°C |
| |
R: 5′-TTCCTGTTGTTTCTATTTCCTTTGT-3′ |
|
| HO-1 |
F: 5′-TGAAGGAGGCCACCAAGGAGG-3′ |
62°C |
| |
R: 5′-AGAGGTCACCCAGGTAGCGGG-3′ |
|
| β-actin |
F: 5′-ATGAAGATCCTGACCGAGCGT-3′ |
58°C |
| R: 5′-AACGCAGCTCAGTAACAGTCCG-3′ |
F, forward; R, reverse.