Schema of the PpCCD4 allele structures and related encoded proteins.
Different colors for the coding regions are reflective of distinct sequence haplotypes. The microsatellite region and the stop codon are indicated in green and red, respectively. The inverted black triangle and the red diamond indicate the positions of conserved glutamates (E)/aspartates (D) and histidines (H) residues on the protein, respectively.
(a) A putatively functional form of the gene with a (TC)7/10 repeat, encoding a complete protein.
(b) Haplotype presenting eight dinucleotide TC repeats that causes a truncated protein.
(c) Ancestral TC7 haplotype prior to occurrence of the nonsense mutation.
(d) Haplotype with (TC)7 microsatellite and an SNP at nucleotide position 1519 causing a premature stop codon, resulting in an incomplete protein lacking a histidine and glutamate residues.
(e) Haplotype with (TC)7 microsatellite and an intronic retroelement (RE) insertion. A schematic diagram of a non-autonomous Copia-like retrotransposon (5282 bp) with putative primer binding site (PBS), polypurine tract (PPT) and long terminal repeats (LTR) is shown.