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. 2014 Jul 21;9:144. doi: 10.1186/1746-1596-9-144

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Copyright © 2014 Rimsza et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Non-specific nucleolar staining with lambda probe. A-C: Germinal center in a reactive tonsil. (A) polyclonal mixture of KAPPA and LAMBDA positive cells (400×); (B&C): arrows point out KAPPA expressing cells as evidenced by black ring of cytoplasmic staining with non-specific pink nucleolar staining (600×). D-E: Follicular lymphoma. (D) low power view of FL case which was indeterminate for KAPPA and lambda due to expressing neither light chain (40×); (E): high power view of same case demonstrating lack of cytoplasmic staining pattern for either KAPPA or LAMBDA light chain expression with non-specific pink nucleolar staining (600×).