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. 2014 Jul 15;7:48. doi: 10.1186/s13045-014-0048-6

Table 2.

Primers for JAK2 Exon 12, JAK2 V617F and CALR mutation screening and sequencing

  Primer Sequence (5’-3’) Amplicon length (bp)
JAK2 exon 12 mutation screening
E12HRM-F
AATGGTGTTTCTGATGTACC
127
 
E12HRM-R
AGACAGTAATGAGTATCTAATGAC
 
JAK2 exon 12 sequencing
E12SEQ-F
CTCCTCTTTGGAGCAATTCA
496
 
E12SEQ-R
GAGAACTTGGGAGTTGCGATA
 
JAK2 V617F
V617F-F
AGCTTTCTCACAAGCATTTGG
150
 
V617F-R
TGACACCTAGCTGTGATCCTG
 
 
V617F-P
AAATTATGGAGTATGTTTCTGTGGAGACGAGA
 
CALR exon sequencing
CALRExon1-F
CGGGTGGGTATAAAAGTG
348
 
CALRExon1-R
GGGACGCAGAAGAGAAAT
 
 
CALRExon2-F
GTTGGAATGGGGAGTGTC
337
 
CALRExon2-R
CTTCCTCCACCTGTCCTC
 
 
CALRExon3-F
CGGTGACGAGGAGAAAGATA
493
 
CALRExon3-R
TAAGAAAGTCAATGGGGTCT
 
 
CALRExon4.5-F
GTTTCTCTTCTCAGCCTTG
678
 
CALRExon4.5-R
TCTCTTCATCCCAGTCCTC
 
 
CALRExon6.7-R
ATCCCACAGACTCCAAGC
611
 
CALRExon6.7-R
GGACTTCACAGGGACAGAC
 
 
CALRExon8.9-F
CGGTGTTCCTTGTCTTCTC
584
  CALRExon8.9-R ACAGAGACATTATTTGGCG