Fig. 2.

Targeted determination of proximal, distal, and transient protein interactors of the yeast nuclear pore complex protein Nup84. Protein A-tagged Nup84 was affinity-isolated in the absence or presence of the stabilizing reagent glutaraldehyde (see Fig. 1). The bars provide the percentage sequence coverage of affinity-isolated protein interactors in the absence (dark) and presence (light) of glutaraldehyde stabilization. For the same experiments, comparable results were obtained when the amounts of affinity captured proteins were estimated by spectral counting (supplemental Fig. S1).