Fig. 4.

Comparison of the proteome resulting from affinity co-capture using protein A-tagged MCM subunits in (–)-glutaraldehyde (light) and (+)-glutaraldehyde (dark) experiments. Peptide MS intensities for each type of experiment were binned together according to the abundance of the corresponding protein (MS intensities for the tagged MCM subunits were excluded). The protein abundances were estimated using codon adaptation indexes (CAI) (2). The results indicate that glutaraldehyde treatment provides stabilization of the specific interactors without a significant increase of highly abundant, nonspecific background.