Fig. 2.

The four-stage scheme for integrative structure determination of the SEA complex. Our integrative approach proceeds through four stages (22, 28–31): (i) gathering of data, (ii) representation of subunits and translation of data into spatial restraints, (iii) configurational sampling to produce an ensemble of models that satisfy the restraints, and (iv) analysis and assessment of the ensemble. The modeling protocol (i.e. Stages 2, 3, and 4) was scripted using the Python Modeling Interface, version 47dafcc, a library to model macromolecular complexes based on our open source Integrative Modeling Platform package, version 65734ec (32).