Tul1 and Dsc3 expression requires E3 ligase subunits.
A, microsomal extracts from wild-type, tul1Δ, dsc2Δ, dsc3Δ, and ubx3Δ cells immunoblotted with anti-Tul1, anti-Dsc2, anti-Dsc3, and anti-Ubx3 IgG. B, microsomal extracts from wild-type, tul1Δ, dsc2Δ, dsc3Δ, and ubx3Δ cells were treated in the presence or absence of PNGase F and immunoblotted with anti-Tul1 IgG. C, indicated strains were treated with proteasome inhibitor MG-132 (100 μm) or dimethyl sulfoxide for 2 h. Microsomal extracts were prepared and immunoblotted with anti-Tul1 and anti-Dsc3 IgG. D, microsomal extracts from the indicated strains were prepared and immunoblotted with anti-Tul1 and anti-Dsc3 IgG.