Fig. 4.

Tul1 E3 ligase complex displays genetic interactions with the multivesicular body pathway. A, indicated strains expressing GFP-tagged mutant form of Pep12 (Pep12D) were imaged using fluorescence microscopy. Staining of vacuolar lumen or limiting membrane is visible. B, indicated single and double mutant yeast strains were spotted on YPD rich medium at 37 °C and grown for 2 days. C, 5-fold serial dilutions of indicated single and double mutants carrying empty vector (pRS426) or expressing 6HIS-myc-ubiquitin (pUB221) were spotted on YPD rich medium with no added copper at 30 °C or 37 °C and grown for 2 days.